Pre-spotted assay plates are available in several content options. They allow for excellent flexibility in the number of samples and targets compared to other formats. Pre-spotted assay plates from Thermo Fisher Scientific are optimal for medium-sized projects involving more than 10 samples and/or fewer than 48 assay targets. Additionally, since the position of each target on the plate is already known, it is possible to download plate setup information from TaqMan Files and have it ready in advance. This is ideal for minimizing sample waste and standardizing assay volume for additional precision, and it also streamlines data analysis. Adding only one mixture to the plate can be sped up dramatically using multi-channel pipettes or even fully automated solutions. In this scenario, a single combination of sample and master mix is sufficient. With pre-spotted plates, however, each well is already seeded with its corresponding assay target or reference gene. Such a complex setup is difficult to automate or mechanize, requiring manual assembly to complete. This setup requires 32 different combinations of target assay, reaction master mix, and sample, creating many opportunities for user error and reagent waste. The targets and a single housekeeping/reference gene are run in triplicate, completing 96 wells on a single plate and 10 plates in total. Pre-spotted plates are an alternative worth considering as they can dramatically reduce the labor and possibility for error of running complex qPCR experiments.įor example, imagine a qPCR experiment consisting of 10 samples and 31 assay targets. For complex qPCR experiments involving multiple genes, however, 96-well plates can rapidly become daunting. Even alternatives to the standard plate, such as tube strips, are designed to emulate the 96-well form factor. The most common format for qPCR is the 96-well plate.

Download lunatic 96 well plate